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ASTM D7463-2008 水中微生物的三磷酸腺甙(ATP)含量的标准试验方法
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【英文标准名称】: Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
【原文标准名称】: 水中微生物的三磷酸腺甙(ATP)含量的标准试验方法
【标准号】: ASTM D7463-2008
【标准状态】: 现行
【国别】: 美国
【发布日期】: 2008
【实施或试行日期】:
【发布单位】: 美国材料与试验协会(US-ASTM)
【起草单位】: D02.14
【标准类型】: (Test Method)
【标准水平】: ()
【中文主题词】:
【英文主题词】: adenosine triphosphate; ATP; bacteria; biocontamination; biodeterioration; biomass; fuel; fungi; microbe; microbiology
【摘要】: This test method measures the concentration of ATP present in the sample. ATP is a constituent of all living cells including bacteria and fungi. Consequently, the presence of ATP is a reliable indicator of microbial contamination in fuel systems. ATP is not associated with matter of non-biological origin. This test method differs from Test Method D 4012 as follows: By providing for the rapid determination of ATP present in a fuel (petroleum) sample, a fuel and water mixture sample, fuel-associated bottom water sample and extracellular ATP freely available in the fuel or aqueous sample matrix; By providing for a method to capture, extract and quantify ATP using self-contained test device and luminometer; By providing a method of quantifying ATP present in fuel or water matrices in generally less than 10 min; and By providing for the rapid separation of the ATP from chemical interferences that have previously prevented the use of ATP determinations in complex fluids containing hydrocarbons and other organic molecules. This test method does not require the use of hazardous materials and does not generate biohazard waste. This test method can be used to estimate viable microbial biomass, to evaluate the efficacy of antimicrobial pesticides, and to monitor microbial contamination in fuel storage and distribution systems.1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with: 1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40x00B0;C) of x2264; 8 mm2 x00B7; sx2013;1 as described in Table X6.1, 1.1.2 Microorganisms found in fuel-associated bottom water, and 1.1.3 Extracellular (non-cellular) ATP present in the sample matrix. 1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens and a dedicated luminometer and reported in (instrument specific) Relative Light Units. 1.3 This test method is equally suitable for use in the laboratory or field. 1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi. 1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 x00D7; 10x2013;11 M to 3 x00D7; 10x2013;8 M which is equivalent to 1 x00D7; 10x2013;14 moles/mL to 3 x00D7; 10x2013;11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 x00D7; 10x2013;11 M to 2 x00D7; 10x2013;14 M. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all o......
【中国标准分类号】: Z16
【国际标准分类号】: 75_160_20
【页数】: 7P.;A4
【正文语种】: 英语

关键字: ASTM D7463-

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